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anti neun primary antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti neun primary antibody
    Anti Neun Primary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+neun+primary+antibody/pmc12721196-126-6-10
    Average 86 stars, based on 1 article reviews
    anti neun primary antibody - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: c-Abl Tyrosine Kinase-Mediated Neuronal Apoptosis in Subarachnoid Hemorrhage by Modulating the LRP-1-Dependent Akt/GSK3β Survival Pathway.
    Article Snippet: Accumulating evidence suggests that neuronal apoptosis plays a critical role in early brain injury (EBI) after subarachnoid hemorrhage (SAH), and the inhibition of apoptosis can induce neuroprotective effects in SAH animal models. c-Abl has been reported to promote neuronal apoptosis in Alzheimer’s disease and cerebral ischemia, but its role in SAH had not been illuminated until now.. In the present study, the effect of c-Abl on neuronal apoptosis induced by SAH was investigated. c-Abl protein levels and neuronal apoptosis were markedly increased 24 h after SAH, and the inhibition of endogenous c-Abl reduced neuronal apoptosis and mortality and ameliorated neurological deficits.. Furthermore, c-Abl inhibition decreased the expression of cleaved caspase-3 (CC-3) after SAH.

    Article Title: A special cranial nucleus (CSF-contacting nucleus) in primates
    Article Snippet: .. The CB stained sections were incubated with anti-NeuN primary antibody (1:50 dilution, CST) at 4 °C overnight, followed by incubation with donkey anti-rabbit Alexa Fluor 488 secondary antibody (1:200 dilution, Life Technologies). ..

    Article Title: Tremella fuciformis polysaccharides alleviate early brain injury in experimental subarachnoid hemorrhage by inhibiting the KDR-mediated P38 MAPK/NF-κB pathway
    Article Snippet: 2.12 Terminal deoxynucleotidyl transferase–mediated dUTP nick end labelling In accordance with the manufacturer's guidelines, TUNEL staining is conducted on frozen brain sections utilizing the TUNEL detection kit (C1089, Beyotime, Nanjing, China). .. The protocol entails the following steps: Initially, the slides are incubated with anti-NeuN primary antibody (1:200, 24307, Cell Signaling Technology) overnight at 4°C to ensure adequate binding to NeuN protein. ..

    Article Title: PHGDH-dependent serine metabolism in astrocytes: A key regulator of oxidative stress and pyroptosis in cerebral ischemia-reperfusion injury.
    Article Snippet: To detect neuronal apoptosis, TdT-mediated dUTP nick-end labeling (TUNEL) staining was performed with a One-Step TUNEL Assay Kit (Beyotime). .. Brain slices were first incubated with anti-NeuN primary antibody (1:300, CST) at 4 ◦C overnight. ..

    Article Title: c-Abl Tyrosine Kinase Mediated Neuronal Apoptosis in Subarachnoid Hemorrhage by Modulating the LRP-1-Dependent Akt/GSK3β Survival Pathway
    Article Snippet: .. Brie y, frozen sections were rewarmed at room temperature for 20 min, blocked with 5% sheep serum(Equitech-Bio,SS-0100) for 1 h and then incubated with anti-NeuN primary antibody (Cell Signaling Technology, cat# 12943, 1:200 dilution). .. A TUNEL kit (Roche, cat# 11684795910) was used to label apoptotic cells after the use of anti-NeuN secondary antibody (Alexa Fluor® Plus 594-conjugated).

    Article Title: PHGDH-dependent serine metabolism in astrocytes: A key regulator of oxidative stress and pyroptosis in cerebral ischemia-reperfusion injury
    Article Snippet: To detect neuronal apoptosis, TdT-mediated dUTP nick-end labeling (TUNEL) staining was performed with a One-Step TUNEL Assay Kit (Beyotime). .. Brain slices were first incubated with anti-NeuN primary antibody (1:300, CST) at 4 °C overnight. ..

    Staining:

    Article Title: A special cranial nucleus (CSF-contacting nucleus) in primates
    Article Snippet: .. The CB stained sections were incubated with anti-NeuN primary antibody (1:50 dilution, CST) at 4 °C overnight, followed by incubation with donkey anti-rabbit Alexa Fluor 488 secondary antibody (1:200 dilution, Life Technologies). ..

    Binding Assay:

    Article Title: Tremella fuciformis polysaccharides alleviate early brain injury in experimental subarachnoid hemorrhage by inhibiting the KDR-mediated P38 MAPK/NF-κB pathway
    Article Snippet: 2.12 Terminal deoxynucleotidyl transferase–mediated dUTP nick end labelling In accordance with the manufacturer's guidelines, TUNEL staining is conducted on frozen brain sections utilizing the TUNEL detection kit (C1089, Beyotime, Nanjing, China). .. The protocol entails the following steps: Initially, the slides are incubated with anti-NeuN primary antibody (1:200, 24307, Cell Signaling Technology) overnight at 4°C to ensure adequate binding to NeuN protein. ..



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    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
    Primary Antibodies Against Neun, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
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    Servicebio Inc primary antibody 178 against neun
    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
    Primary Antibody 178 Against Neun, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Novus Biologicals anti neun primary antibody
    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
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    Image Search Results


    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and NeuN (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.

    Journal: bioRxiv

    Article Title: Super-resolved, three-dimensional spatial transcriptomics reveals cell-type and brain-region-specific modulation of key epitranscriptomic switches following adolescent alcohol exposure

    doi: 10.64898/2026.01.22.698893

    Figure Lengend Snippet: (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and NeuN (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.

    Article Snippet: Primary antibodies against NeuN [guinea pig, 266004, Synaptic Systems (SYSY)], GFAP (guinea pig, 173308, SYSY), or IBA1 (chicken, 234009, SYSY) were used to label cells of each cell type in the sample ( Supplemental Table S1 ).

    Techniques:

    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the BLA of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the BLA of AIE rats. All the images in (a-c) are MIP images (∼8.6 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.990), (e) astrocytes ( p = 0.371), and (f) microglia ( p = 0.792) in the BLA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

    Journal: bioRxiv

    Article Title: Super-resolved, three-dimensional spatial transcriptomics reveals cell-type and brain-region-specific modulation of key epitranscriptomic switches following adolescent alcohol exposure

    doi: 10.64898/2026.01.22.698893

    Figure Lengend Snippet: (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the BLA of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the BLA of AIE rats. All the images in (a-c) are MIP images (∼8.6 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.990), (e) astrocytes ( p = 0.371), and (f) microglia ( p = 0.792) in the BLA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

    Article Snippet: Primary antibodies against NeuN [guinea pig, 266004, Synaptic Systems (SYSY)], GFAP (guinea pig, 173308, SYSY), or IBA1 (chicken, 234009, SYSY) were used to label cells of each cell type in the sample ( Supplemental Table S1 ).

    Techniques: Labeling

    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CA1 of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels, with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) in the CA1 and (c) IBA1-positive microglia (white arrows) in the DG of AIE rats. All the images in (a-c) are MIP images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.009), (e) astrocytes ( p = 0.174), and (f) microglia ( p = 0.421) in the hippocampus of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). Individual data points are the average of 9 cells, 3 each from the CA1, CA3, and DG. In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

    Journal: bioRxiv

    Article Title: Super-resolved, three-dimensional spatial transcriptomics reveals cell-type and brain-region-specific modulation of key epitranscriptomic switches following adolescent alcohol exposure

    doi: 10.64898/2026.01.22.698893

    Figure Lengend Snippet: (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CA1 of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels, with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) in the CA1 and (c) IBA1-positive microglia (white arrows) in the DG of AIE rats. All the images in (a-c) are MIP images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.009), (e) astrocytes ( p = 0.174), and (f) microglia ( p = 0.421) in the hippocampus of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). Individual data points are the average of 9 cells, 3 each from the CA1, CA3, and DG. In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

    Article Snippet: Primary antibodies against NeuN [guinea pig, 266004, Synaptic Systems (SYSY)], GFAP (guinea pig, 173308, SYSY), or IBA1 (chicken, 234009, SYSY) were used to label cells of each cell type in the sample ( Supplemental Table S1 ).

    Techniques: Labeling